The Identification of Genetic Material in Bacteria, Study notes of Biotechnology

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In the 1940s, American physician Oswald!Avery and American biologists ColinMacleod and Maclyn McCarty were also interested in the process of bacterial transformation. During the course of their studies, they realized that Griffith's observations could be used as part of an experimental strategy to biochemically identify the genetic materia. They asked the question “Whatisubstance is being transferred from the dead type S bacteria to the live type R bacteria?” To answer this question, Avery, MacLeod, and McCarty needed to purify the general categories of substances found in living cells. eireptococealsirain. Initially, they discovered that only the purified DNA could convert type R bacteria into type S. HYPOTHESIS func eg KEY MATERIALS 7) +DNA JbuuL To further verify that DNA is the genetic material, they performed the TaN =Sod Le FVUCO) PM Dol =) os YUL abi Vo UD) VN Tero gg Nilo O ayo) SSN oF To Td Tale a rol with type R bacteria(step 1). After allowing time for DNA uptake into the type R bacteria, they added an antibody that aggregated any nontransformed type R bacteria, which were then removed bylcentritigatian. The remaining bacteria were incubated overnight on petri plates