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Rapid Microbial Methods - Applied Microbiology - Lecture Slides, Slides of Microbiology

This course includes emerging and reemerging diseases, public health issues and nanotechnology aspects of microbiology and other topics mainly. Main concepts explained in this lectures are: Rapid Microbial Methods, Traditional Microbial Methods, Robert Koch and Pure Cultures, Walter Hesse and Agar, Polysaccharide, Richard Petri, Martinus Beijerinck and Enrichment Culture, Microscopy and Staining Techniques, Gram Stain, Spore Stain

Typology: Slides

2012/2013

Uploaded on 08/30/2013

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IdentificationandCharacterization
Techniques
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Download Rapid Microbial Methods - Applied Microbiology - Lecture Slides and more Slides Microbiology in PDF only on Docsity!

Recent

Advances

in

Rapid

Microbial

Identification

and

Characterization

Techniques

Traditional

Microbial

Methods

Robert

Koch

and

Pure

Cultures

His study of infectious diseases lead to the rise of pure cultures Started using potato slices Gelatin: solution for potato slices

Traditional

Microbial

Methods

Richard

Petri

Double sided clear petri plates Easy to stack, sterilize, view colonies

Traditional

Microbial

Methods

Martinus

Beijerinck

and

Enrichment

Culture

Manipulated incubation and nutrient conditions of natural samples Was able to isolate and identify the first pure cultures of soil and aquatic microorganisms

Lactobacillus species - green algae Was the first to describe a virus - Tobacco mosaic virus

Microscopy

and

Staining

Techniques

Viability staining Fluorescent staining using

DAPI

diamido

phenylindole)

What

Are

Rapid

Microbial

Methods

(RMM)?

Technologies

that

allow

users

to

get

microbial

test

results

faster

than

traditional

methods

Can

be

used

for

qualitative,

quantitative

or

identification Common

Technologies

Used

**Just list the ones we are going to talk about

i will** mention more but say “our focus is...”

Immunoassays

Advantages

Easy to use

Cost effective - Fast detection

hour to

minutes)

Disadvantages

Low sensitivity

Better to use heavily contaminated waters and larger size pathogens - Not successful in detecting enteroviruses

ELISA:

Enzyme

Linked

ImmunoSorbant

Assay

Purpose:
to
determine
if
a
particular
protein
or
target Microtiter
Plates
Used
Forms

Direct - Indirect

Indirect

Elisa

Used

to

detect

presence

of

specific

antibody

in

specimen

 Initial step where Antigen is bound to surface of plate  Sample added  Final step where substrate for enzyme is added  Colour change indicates sample contains antibody that reacts against antigen (Left well)

Immunoliposome

Technology

Involves

things

Liposomes with encapsulated dyes

Antibodies to antigen of microbe tagged outside

Immunostrip

Kit

by

Agida

Inc.

Respiring

Microbe

Method:

ATP

Production

Excellent
way
of
measuring
presence
of
microbes
in
recreational
contaminated
waters
Advantage

fast, simple

Disadvantage

Careful calibration for sample needed

LuminUltra
Technology

Fast - Portable - Complete

Benefits

and

Drawbacks

of

RMM

Benefits

Faster time - Allows companies to release finished products to the market in less time - Ability to identify, contain and recover from contaminant events - Reduce worker subjectivity - Take guesswork out of plating and waiting - Less training - Ease of use - Higher specificity/sensitivity Drawbacks - Can’t replace traditional methods - Cost per test high - Complex technology

Saponification

of

cellular

lipids

and

Fatty

acid

methylation

Triglyceride Methanol Glycerol FAMEs

Saponification

of

cellular

lipids

and

Fatty

acid

methylation

Triglyceride Methanol Glycerol FAMEs