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This course includes emerging and reemerging diseases, public health issues and nanotechnology aspects of microbiology and other topics mainly. Main concepts explained in this lectures are: Rapid Microbial Methods, Traditional Microbial Methods, Robert Koch and Pure Cultures, Walter Hesse and Agar, Polysaccharide, Richard Petri, Martinus Beijerinck and Enrichment Culture, Microscopy and Staining Techniques, Gram Stain, Spore Stain
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His study of infectious diseases lead to the rise of pure cultures Started using potato slices Gelatin: solution for potato slices
Double sided clear petri plates Easy to stack, sterilize, view colonies
Manipulated incubation and nutrient conditions of natural samples Was able to isolate and identify the first pure cultures of soil and aquatic microorganisms
Lactobacillus species - green algae Was the first to describe a virus - Tobacco mosaic virus
Viability staining Fluorescent staining using
diamido
phenylindole)
**Just list the ones we are going to talk about
i will** mention more but say “our focus is...”
Easy to use
Cost effective - Fast detection
hour to
minutes)
Low sensitivity
Better to use heavily contaminated waters and larger size pathogens - Not successful in detecting enteroviruses
Direct - Indirect
Initial step where Antigen is bound to surface of plate Sample added Final step where substrate for enzyme is added Colour change indicates sample contains antibody that reacts against antigen (Left well)
Liposomes with encapsulated dyes
Antibodies to antigen of microbe tagged outside
fast, simple
Careful calibration for sample needed
Fast - Portable - Complete
Benefits
Faster time - Allows companies to release finished products to the market in less time - Ability to identify, contain and recover from contaminant events - Reduce worker subjectivity - Take guesswork out of plating and waiting - Less training - Ease of use - Higher specificity/sensitivity Drawbacks - Can’t replace traditional methods - Cost per test high - Complex technology
Triglyceride Methanol Glycerol FAMEs
Triglyceride Methanol Glycerol FAMEs