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The University of Texas at Tyler
Environmental Health and Safety
BIOLOGICAL AGENT REFERENCE SHEET
Risk Group 2 - Agents that are associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available. Agent Type Biohazard Providencia rettgeri is a Gram-negative, motile, rod-shaped bacterium. They are found ubiquitously in natural environments. P. rettgeri causes different disease in humans depending on the route of exposure; many of these exposures go undocumented. However, there is a large interest in its use as a eukaryotic pathogen for pest control. This organism has sporatic antibiotic resistance to some modern mediciations. ref: A Case of Wound Infection with Providencia rettgeri and Coincident Gout in a Patient from Guam. Hawaii J Med Public Health. 2015; Isolation of insect pathogenic bacteria, Providencia rettgeri , from Heterorhabditis spp. Host Range Humans; animals Exposure route Aerosol/inahalation; mucous membrane contact Incubation period unknown High Energy Centrifugation, sonication, vortexing
Characteristics
Description
Laboratory Hazards
Sharps Aerosols Shaking, liquid culturing, pipetting, coughing, sneezing Equipment Can adhere to laboratory equipment from 24 hours to 120 days Exposed body oral and nasal respiratory tracts; Notes unknown
Biosafety Level 2 - refer to Biosafety Manual; contact EH&S for a copy
Training EH&S Biosafety Training; Lab specific training Engineering controls use in BSL II only PPE Eye protection, gloves and lab coat Waste Biohazard - put in red biohazard bins
Disinfection 10% bleach; 70% ethanol
Survival outside host Engineering controls BSC; lids while working with high energy equipment PPE Eye protection, gloves, long sleeve or lab coat Waste Biohazard - put in red biohazard bins
Mucous membranes flush eyes, nose, mouth/throat for 15 minutes
Skin contact Wash with soap and water for a minimum of 30 second for bare skin contact; for broken skin wash with soap and water for 15 minutes Minor (small) spills Notify all persons present in the area. Allow aerosols to settle. While wearing protective clothing, gently cover the spill with absorbent paper towel and apply appropriate disinfectant, starting at perimeter and working towards the centre. Allow sufficient contact time before clean up. Major (large) spills Contact EH&S immediately; after-hours contact University Police Waste Decontaminate all wastes before disposal by incineration, chemical disinfection or steam sterilization
Exposure and Spill procedures
Laboratory Handling Guidelines
Agent Viability
