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Optimized Idronoxil Loaded Polycaprolactone for Targeted Liver cancer therapy, Study Guides, Projects, Research of Pharmacy

Saveetha Institute of Medical and Technical SciencesPharmacy

This study presents the development and optimization of Idronoxil-loaded polycaprolactone (PCL) nanoparticles for targeted treatment of liver cancer (Hepatocellular Carcinoma, HCC). Idronoxil, a synthetic flavonoid with known anticancer properties, was encapsulated within biodegradable PCL using the ionic gelation technique. The nanoparticles were characterized using FTIR, DSC, SEM, DLS, and HPLC to confirm drug-polymer compatibility, stability, morphology, particle size (~97.3 nm), zeta potential (–6.41 mV), and high encapsulation efficiency (82.07%). In vitro drug release studies demonstrated sustained drug release over 24 hours, and MTT assays on HepG2 cells showed potent cytotoxicity (IC₅₀ = 19.33 µg/mL). The formulation remained stable over 90 days under accelerated conditions. These findings suggest that this nanoparticle system holds strong potential for improved liver cancer therapy with reduced toxicity and enhanced efficacy.

Typology: Study Guides, Projects, Research

2024/2025

Available from 06/03/2025

thara-venkatesh
thara-venkatesh 🇮🇳

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Optimized Idronoxil-Loaded
Polycaprolactone Nanoparticles for
Targeted Liver Cancer Therapy: A Novel
Approach in Drug Delivery Systems
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Download Optimized Idronoxil Loaded Polycaprolactone for Targeted Liver cancer therapy and more Study Guides, Projects, Research Pharmacy in PDF only on Docsity!

Optimized Idronoxil-Loaded

Polycaprolactone Nanoparticles for

Targeted Liver Cancer Therapy: A Novel

Approach in Drug Delivery Systems

Table of Contents

  • (^) Introduction
  • (^) Objective
  • (^) Materials Used
  • (^) Methodology – Ionic Gelation Process
  • Characterization Techniques
  • (^) FTIR & DSC Compatibility Studies
  • (^) Particle Size, SEM & Zeta Potential
  • (^) Encapsulation Efficiency & Drug Content
  • (^) In Vitro Drug Release Study
  • (^) Stability Testing
  • (^) In Vitro Cytotoxicity (MTT Assay)
  • (^) Results Summary
  • (^) Discussion
  • (^) Conclusion
  • (^) Future Scope
  • (^) Bibliography

Objective

  • (^) Formulate Idronoxil-loaded PCL nanoparticles.
  • (^) Optimize them for size, zeta potential, and encapsulation efficiency.
  • (^) Characterize their physicochemical properties.
  • (^) Evaluate cytotoxic potential against HepG2 liver cancer cells.

Materials Used

Idronoxil (active drug),

  • (^) PCL (polymer),
  • (^) PVA (surfactant),
  • (^) TPP (crosslinker) were the key materials.

Characterization

Techniques

  • (^) FTIR : Drug–polymer compatibility.
  • (^) DSC : Thermal stability and phase transition.
  • (^) SEM : Surface morphology and shape.
  • (^) DLS : Particle size, Zeta potential.
  • (^) HPLC : Drug content and encapsulation efficiency.

FTIR & DSC

Compatibility

FTIR showed no new peaks → no chemical interaction.

  • (^) DSC showed separate thermal peaks for drug and polymer.
  • (^) Confirms physical entrapment, not chemical bonding.

Encapsulation

Efficiency & Drug

Content

EE: 82.07%, indicating effective drug entrapment.

  • (^) High EE reduces drug wastage and ensures sustained availability.
  • (^) HPLC validated drug content using a standard method at 247 nm.

In Vitro Drug Release

Drug release was studied in phosphate buffer (pH 7.4).

  • (^) Sustained and controlled release observed up to 24 hrs.
  • (^) Helps reduce dosing frequency and enhances therapeutic window.

In Vitro Cytotoxicity

(MTT Assay)

HepG2 liver cancer cells used.

  • (^) IC50 = 19.33 μg/mL indicates potent cytotoxicity.
  • Cell death increased in a dose- dependent manner.
  • (^) Suggests strong anti-liver cancer potential of the formulation.

Results Summary

Optimized formulation: 97.3 nm, –6.41 mV, 82.07% EE.

  • (^) Sustained drug release, high stability, strong anticancer activity.
  • (^) Statistical design successfully predicted ideal conditions.

Conclusion

Successfully developed and optimized Idronoxil-loaded PCL nanoparticles.

  • (^) Demonstrated ideal properties for drug delivery in liver cancer.

Future Scope

In vivo animal studies to confirm efficacy and pharmacokinetics.

Bibliography

  • (^) Ghasemi, M., et al. (2021). The MTT assay: Use, limitations, and interpretation. International Journal of Molecular Sciences , 22(23), 12827.
  • (^) Nga, N. T., et al. (2020). Optimization of MTT assay for suspension cells. Analytical Biochemistry , 610, 113937.
  • (^) Kessaissia, F. Z., et al. (2020). Factorial design optimization in PV modules. Energy Reports , 6, 299–309.
  • (^) Tkachenko, Y., & Niedzielski, P. (2022). FTIR in solid sample assessment. Molecules , 27(24), 8846.
  • (^) Budiman, A., et al. (2023). Amorphous solid dispersions for drug delivery. Polymers , 15(16), 3380.
  • (^) Qiu, X. L., et al. (2021). SNEDDS loaded with heparin phospholipid complex. International Journal of Molecular Sciences , 22(8), 4077.
  • (^) Danaei, M. R., et al. (2018). Impact of particle size and PDI in lipidic nanocarriers. Pharmaceutics , 10(2), 57.
  • (^) Agrawal, M., et al. (2021). Curcumin-loaded lipid carriers using Box-Behnken design. Biomedicine & Pharmacotherapy , 141, 111919.
  • (^) Tantra, R., et al. (2010). Effect of nanoparticle concentration on zeta potential. Particuology , 8(3), 279–285.
  • (^) Mohamed, R. M., & Yusoh, K. (2016). Polycaprolactone (PCL) in drug delivery: A review. Advanced Materials Research , 1134, 249–255.
  • (^) Mehmood, A., et al. (2023). Market trends of polylactic acid biopolymers. Materials Today: Proceedings , 72, 3049–3055.
  • (^) Bai, X., et al. (2022). Smart nanoparticles for sustained drug release in cancer. Micromachines , 13(10), 1623.
  • (^) Augustine, R., et al. (2020). Cellular uptake and retention of nanoparticles. Materials Today Communications , 25, 101692.

Thank You