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AGRICULTURAL SCIENTISTS RECRUITMENT BOARD
INDEX
Syllabus for ARS/NET Examination
- KRISHI ANUSANDHAN BHAWAN‐ 1 PUSA NEW DELHI ‐
- Agril. Biotechnology 01 ‐ Code No. Discipline Page No.
- Agril. Entomology 03 ‐
- Agril. Microbiology 07 ‐
- Economic Botany & Plant Genetics Resources 09 ‐
- Genetics & Plant Breeding 11 ‐
- Nematology 13 ‐
- Plant Biochemistry 15 ‐
- Plant Pathology 18 ‐
- Plant Physiology 20 ‐
- Seed Science & Technology 24 ‐
- Floriculture & Landscaping 26 ‐
- Fruit Science 29 ‐
- Spices, Plantation & Medicinal & Aromatic Plants 32 ‐
- Vegetable Science 34 ‐
- Animal Biochemistry 37 ‐
- Animal Biotechnology 39 ‐
- Animal Genetics & Breeding 41 ‐
- Animal Nutrition 44 ‐
- Animal Physiology 46 ‐
- Animal Reproduction & Gynaecology 48 ‐
- Dairy Chemistry 51 ‐
- Dairy Microbiology 54 ‐
- Dairy Technology 57 ‐
- Livestock Product Technology 59 ‐
- Livestock Production Management 61 ‐
- Poultry Science 64 ‐
- Veterinary Medicine 67 ‐
- Veterinary Microbiology 70 ‐
- Veterinary Parasitology 72 ‐ Code No. Discipline Page No.
- Veterinary Pathology 74 ‐
- Veterinary Pharmacology 76 ‐
- Veterinary Public Health 78 ‐
- Veterinary Surgery 81 ‐
- Aquaculture 83 ‐
- Fisheries Resource Management 85 ‐
- Fish Process Technology 87 ‐
- Fish Nutrition
- Fish Health 90 ‐
- Fish Genetics & Breeding
- Agricultural Chemicals 93 ‐
- Agricultural Meteorology 96 ‐
- Agroforestry 98 ‐
- Agronomy 101 ‐
- Environmental Sciences 104 ‐
- Soil Sciences 107 ‐
- Agricultural Business Management 109 ‐
- Agricultural Economics 112 ‐
- Agril. Extension 115 ‐
- Agril. Statistics 118 ‐
- Home Sciences 121 ‐
- Farm Machinery and Power 124 ‐
- Agricultural Structures and Environmental Management 126 ‐
- Land and Water Management Engineering 129 ‐
- 54 Agricultural Process Engineering 131 ‐
- Food Technology 133 ‐
Ribosome structure and function. Protein biosynthesis in prokaryotes and ekaryotes. Post-translational modification. Gene regulation, RNA processing and Post transcriptional modifications. Bioprospecting, biofortification, gene pryrimiding and gene fusion, nbozyme technology.
Unit 9: Plant Molecular Biology Photoregulation and phytochrome regulation of nuclear and chloroplastic gene expression. Molecular mechanism of nitrogen fixation. Molecular biology of various stresses, viz. abiotic stresses like drought, salt, heavy metals and temperature; and biotic stresses like bacterial, fungal and viral diseases. Signal transduction and its molecular basis, molecular mechanism of plant hormone action mitochondrial control of fertility, structure, organization and regulation of nuclear gene concerning storage proteins and starch synthesis.
Unit 10: Tissue Culture Basic techniques in cell culture and somatic cell genetics. Regulation of cell cycle and cell division.. Clonal propagation. Concept of cellular totipotency. Anther culture, somaclonal and gametoclonal variations. Hybrid embryo culture and embryo rescue, somatic hybridization and cybridization. Application of tissue culture in crop improvement. Secondary metabolite production. In vitro , mutagenesis, cryopreservation and plant tissue culture repository.
Unit 11: Plant Genetic Engineering Isolation of genes of economic importance. Gene constructs for tissue-specific expression. Different methods of gene transfer to plants, viz. direct and vector- mediated. Molecular analysis of transformants. Potential applications of plant genetic engineering for crop improvement, i.e. insect-pest resistance (insect, viral, fungal and bacterial disease resistance), abiotic stress resistance, herbicide resistance, storage protein quality, increasing shelf-life, oil quality, Current status of transgenics, biosafty norms and controlled field trials and release of transgenics (GMOs).
Unit 12: Molecular Markers and Genomics DNA molecular markers: Principles, type and applications; restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA sequences (RAPD), Simple sequence repeats (SSR), Single nucleotide polymorphism (SNP), Structural and functional genomics, gene mapping, genome mapping, gene tagging and comparative genomics and application of genomics.
02. AGRICULTURAL ENTOMOLOGY
Unit 1: Systematics History and development of Entomology, Evolution of insects, position of insects in the animal world, characteristics of phylum Arthropoda, structural features of important arthropod groups such as Trilobita, Chelicerata and Mandibulata, structural features of important classes of phylum Arthropoda viz. Arachnida, Crustacea, Chilopoda, Diplopoda and Hexapoda. Classification of insects up to order level, habits, habitats and distinguishing features of different Order and important Families.
Unit 2: Morphology Body wall, its structure, outgrowths, endoskeleton, Body regions, segmentation, sclerites and sutures. Head and head appendages, types of mouth parts, antennae, their structure and types. Thorax structure, thoracic appendages and their modification. Wings, their modification and venation, Abdomen; structure, abdominal appendages both in Pterygota and Apterygota. External genitalia, general structure and modification in important insect orders.
Unit 3: Embryology, Internal Anatomy and Physiology Embryonic and post embryonic development, types of metamorphosis, physiology of ecdysis. General features and types of larvae and pupae. Structure, function and physiology of Digestive, Circulatory, Respiratory, Reproductive, Nervous and Excretory systems, Sense Organs; structure and types. Insect food and nutrition; minerals, carbohydrates, proteins and amino acids, lipids, vitamins and their role in growth and development, artificial diets.
Unit 4: Ecology Concept of ecology, Environment and its components-biotic and abiotic factors and their effects on growth, development, population dynamics, distribution and dispersal. Principle of biogeography and insects biodiversity. Biotic potential and environmental resistance. Ecosystems, agroecosystems analysis, their characteristics and functioning. Intra and inter specific relationship; competition, predator-prey and host-parasite interactions, ecological niche. Life table studies, population models. Food chain and food web. Arthropod population monitoring, pest forecasting. Diapause and causes of pest out breaks.
Unit 5: Biological Control Importance and scope of biological control, history of biological control: Biocontrol agents-parasites, predators and insect pathogens. Important entomophagous insect Orders and Families. Ecological, biological, taxonomic, legal and economic aspects of biological control, phenomena of multiple parasitism, hyperparasitism, superparasitism and their applied importance. Principles and procedures of using exotic biocontrol agents. Utilization of natural biocontrol agents: conservation, habitat management and augmentation. Mass multiplication techniques and economics. Effective evaluation techniques, Biocontrol organizations in world and India. Successful cases of biological control of pests.
Entomophilic pathogens: bacterial, fungi, viruses, rickettsiae, Protozoan and nematodes, Modes of transmission, methods of uses, symptoms of infection.
Types of appliances: sprayers, dusters, fog generators, smoke generators, soil injecting guns, seed treating drums, flame throwers, etc. Power operated sprayers and dusters. Types of nozzles and their uses. Maintenance of appliances. Aerial application of pesticides, principles of aerial application, factors affecting the effectiveness of aerial application. Equipments for aerial applications. Advantages and disadvantages of aerial application.
Unit 11: Pests of Field Crops and their Management Distribution, host range, biology and bionomics, nature of damage and management of arthropod pests of cereals, Oilseed, pulses and fibre crops, sugarcane and tobacco. Polyphagous pests: locusts, termites, hairy catepillars, cut worms and white grubs.
Unit 12: Pests of Horticultural Crops and their Management Distribution, host range, biology and bionomics, nature of damage and management of arthropod pests of vegetables, fruits and plantation crops, spices, condiments and ornamentals.
Unit 13: Pests of Stored Products and their Management Fundamentals of storage of grains and grain products. Storage losses, sources of infestation/infection, factors influencing losses, insect and non-insect pests, their nature of damage and control. Microflora in storage environment and their control. Storage structures, bulk storage and bag storage, their relative efficacy and demerits. Grain drying methods and aeration. Non-insect pests (rodents, birds, mites) of stored products and their control. Integrated management of storage pests.
Unit 14: Arthrpod Vectors of Plant Diseases Common arthropod vectors viz ., aphids, leaf hoppers, plant hoppers, whiteflies, thrips, psylids, beetles, weevils, flies, bees and mites and their relationship with the plant pathogenic fungi, bacteria, viruses, mycoplasma. Mechanism of pathogen transmission : Active mechanical transmission, biological transmission. Toxicogenic insects, mites and phytotoxemia. Some important arthropod vector transmitted diseases and their epidemiology in India. Management of vector and its effect on control of diseases.
Unit 15: Honey Bees and Bee-keeping Honey bees and their economic importance. Bee species, their behaviour, habit and habitats. Bee Keeping: bee pasturage, hives and equipments, seasonal management. Bee enemies including diseases and their control.
Unit 16: Silkworms and Sericulture Silkworm species, their systematic position and salient features. Rearing techniques of mulberry-muga-eri and tassar silkworms. Nutritional requirements of silkworms. Sericulture: rearing house and appliances, silkworm breeds, principles of voltism and nioultism, seed production and its economics. Enemies and diseases of silkworms and their management. Sericulture organization in India. Unit 17: Lac Insect Lac insect, its biology, habit and habitats. Host Trees: pruning, inoculation, lac cropping techniques, and harvesting. Enemies of lac insect and their control.
Unit 18: Other Useful Insects
Pollinators, biocontrol agents of weeds, soil fertility improving agents, scavengers. Use of insects and insect products in medicines. Usefulness of insects in scientific investigations, insects as food.
Unit 19: Statistics and Computer Application Frequency distribution, mean, mode and median. Standard, normal, bionomial and Poisson’s distribution, Sampling methods and standard errors. Correlation and regression: Partial and multiple, tests of significance; t, F, chi- square, Duncan’s multiple range tests. Design of experiments: Principles of Randomized block design, Completely r andomized block design, Latin square design, Split-plot designs. Probit analysis. Use of soft ware packages like SPSS, SAS, etc. for the above tests and designs of experiments for analysis.
of meat, fish, egg, fruits, vegetables, juices, flour, canned foods; bio- degrading microbes, single cell protein for use as food and feed, bioactive food / probiotics
04. ECONOMIC BOTANY & PLANT GENETIC RESOURCES
Unit 1 : Plant Taxonomy and Biosystematics Nomenclature, purpose, principles and systems of classification; Taxonomy of higher plants, floras, manuals, monographs, index, catalogues and dictionaries, herbaria; Concepts of biosystematics, evolution and differentiation of species; Biosystematic and taxonomic tools; Origin, evolution and biosystematics of selected crops (rice, wheat, rape seed & mustard, cotton).
Unit 2 : Economically important plants –I Origin, history, domestication, botany, genetic resource activities, cultivation, production and use of: Cereals: Wheat, rice, maize, sorghum, pearl millet and minor millets. Pulses: Pigeon pea, chickpea, black gram, green gram, cowpea, soyabean, pea, lentil, horsegram, lab-lab bean, ricebean, winged bean, French bean, lima bean, sword bean. Oilseeds: Groundnut, sesame, castor, rape seed, mustard, sunflower, safflower, niger, oil palm, coconut and linseed.
Unit 3 : Economically important plants –II Origin, distribution, cultivation, production and utilization of economic plants of following groups such as Fibres: cotton, silk cotton, jute, sunnhemp, agave, flax and mesta (kenoff); Sugars: sugarcane, sugarbeet, sugarpalm and sweet sorghum; Fodders and green manure crops: Plantation crops: coconut, cocoa, tea; root and tuber crops-: potato, sweet potato, tapioca, aroids etc.
Unit 4 : Economically important plants –III Origin, distribution, classification, production and utilization of Fruits: mango, banana, citrus, guava, grapes and other indigenous fruits; apple, plum, pear, peach, cashewnut and walnut; Vegetables: tomato, brinjal, okra, cucumber, cole crops, gourds etc.; Fumigatories and masticatories: tobacco, betelvine, areacanut; medicinal and aromatic plants: sarpagandha, belladonna, cinchona, nux-vomica, vinca, mentha and glycirrhiza, plantago etc.; Narcotics: cannabis, datura, gloriosa, pyrethrum and opium; Dye-, tannin-, gum- and resin- yielding plants; Plant of agro-forestory importance: multipurpose trees/shrubs, subabool, Acacia nilotica, poplar, sesbania, neem etc.; non-traditional economic plants: jojoba, guayule, jatropha, carcus etc.
Unit 5 : Biodiversity and Plant Genetic Resources (PGR) Biosphere and biodiversity; plant species richness and endemism; concept and importance of plant genetic resources and its increasing erosion; Centres of origin and diversity of crop plants, domestication, evaluation, bioprospecting; National and International organizations associated with PGR; Convention on Biological Diversity (CBD), recent issues related to access and ownership of PGR, IPR,. PBRs, farmers rights, sui-generis system etc.
Unit 6 : Germplasm Augmentation History and importance of germplasm collection, ecogeographical distribution of diversity, logistics of exploration and collection, use of flora and herbaria, random and selective sampling, genepool sampling in self and cross pollinated species; Concept, importance and ecogeographical considerations of introduction and exchange of plant germplasm; prerequisites conventions and achievements of PGR exchange.
05. Genetics & Plant Breeding
Unit 1: General Genetics and Plant Breeding Mendelian inheritance. Cell structure and division, Linkage, its detection and estimation. Epistasis. Gene concept, allelism and fine structure of gene. Extra chromosomal inheritance. DNA – structure, function, replication and repair. Genetic code. Gene- enzyme relationship. Replication, Transcription and Translation. Gene regulation in prokaryotes and eukaryotes. Nuclear and cytoplasmic genome organization. Spontaneous and induced mutations and their molecular mechanisms. Crop domestication, evolution of crops and centres of diversity. Emergence of scientific plant breeding. Objectives and accomplishments in plant breeding and the role of National and International institutes. Gametogenesis and fertilization. Modes of sexual and asexual reproduction and its relation to plant breeding methodology. Apomixes, incompatibility and male sterility systems and their use in planr breeding.
Unit 2: Economics Botany and Plant Breeding Methods Origin, distribution, classification, description and botany of cereals (wheat, rice, maize, sorghum, pearl millet, minor millets); pulses (pigeonpea, chickpea, black gram, green gram, cowpea, soyabean, pea, lentil, horse gram, lab-lab, rice bean, winged bean, lathyrus, Lima bean; oilseeds (groundnuts, sesamum, castor, rapeseed mustard, sunflower, Niger, linseed); fibers and sugar crops, fodder and green manures; Breeding methods for self-pollinated, cross-pollinated and clonally propagated crops.Component, recombinational and transgressive breeding. Single seed descent. Populations, their improvement methods and maintenance, Hybrid breeding and genetic basis of heterosis. Ideotype breeding. Mutation breeding.
Unit 3: Genome organization and Cytogenetics of Crop Plants Chromosome structure, function and replication. Recombination and crossing over. Karyotype analysis. Banding techniques. In situ hybridization. Special types of chromosomes. Chromosomal interchanges, inversions, duplications and deletions. Polyploids, haploids, aneuploids and their utility. Wide hybridization and chromosomal manipulations for alien gene transfer. Pre-and post- fertilization barriers in wide hybridization. Genome organization and cytogenetics of important crop species- wheat, maize, rice, Brassica, cotton, Vigna, potato and sugarcane. Principles and procedures of genome analysis. Cytogenetic techniques foe gene location and gene transfer, Construction and use of molecular marker based chromosome maps. Comparative mapping and genome analysis.
Unit 4: Quantitative and Biometrical Genetics Quantitative characters. Multiple factors inheritance. Genetic control of polygenic characters. Genetic advance and types of selection and correlated response. Hardy Weinberg law. Linkage disequilibrium. Genetic load. Polymorphism. Breeding value, heritability. Response to selection, correlated response. Estimates of variance components and covariance among relatives. Mating designs with random and inbred parents. Estimation of gene effects and combing ability. Effects of linkage and epistasis on estimation of genetic parameters. Maternal effects. Genotype- environment interactions and stability of performance. Heterosis and its basis. Mating system and mating design- diallel, line X tester, NC-1, NC-II and NC-III designs, approaches to estimate and exploit component of self and cross pollinated crops. Genotype X environment interaction and stability analysis.
Unit 5: Genetic Engineering and Biotechnological Tools in Plant Breeding
Somatic hybridization, micropropagation, somachonal variation in vitro mutagenesis. Artificial synthesis of gene. Genetic and molecular markers, generations of molecular markers and their application in genetic analyses and breeding. Molecular markers in genetic diversity analysis and breeding for complex characters. Gene tagging, QTL mapping and marker aided selection. Genome projects and utilization of sequence formation. Vectors. DNA libraries, DNA fingerprinting, DNA sequencing. Nuclei acid hybridization and immunochemical detection. Chromosome walking, Recombinant DNA technology, Gene cloning strategies. Genetic transformation and transgenics. Antisense RNA, RNAi and micro RNA techniques in crop improvement.
Unit 6: Plant Breeding for Stress Resistance and Nutitional Quality Genetic basis and breeding for resistance to diseases and insect-pests. Breeding for vertical and horizontal resistance to diseases. Genetic and physiological basis of abiotic stress tolerance. Breeding for resistance to heat, frost, flood, drought and soil stresses. Important quality parameters in various crops, their genetic basis and breeding for these traits. Role of molecular markers in stress resistance breeding: MAS, MARS and MABB.
Unit 7: Plant Genetic Resources and their Regulatory System; Varietal Release and Seed Production Plant exploration, germplasm introduction, exchange, conservation, evaluation and utilization of plant genetic resoures. Convention on Biological Diversity and International Treaty on Plant Genetic Resources for Food and Agriculture. Intellectual Property Rights. Biodiversity Act. Plant Variety Protection and Farmers' Rights Act. System of variety release and notification. Types of seeds and seed chain. Seed production and certification.
Unit 8: Statistical Methods and Field Plot Techniques Frequency distribution. Measures of central tendency, probability theory and its applications in genetics. Probabaility distribution and tests of significance. Correlation, linear, partial and multiple regression. Genetic divergence. Multivariate analysis. Design of experiments- basic principles, completely randomized design, randomized block design and split plot design. Complete and incomplete block designs. Augmented design, Grid and honeycomb design. Hill plots, unreplicated evaluation. Data collection and interpretation.
Unit 7 : Nematode Management Principles and methods of nematode management - physical, cultural biological, chemical and legislative, Nematicides (including those of biological origin) - history, classification, formulations, application and mode of action. Host resistance for nematode management. Integrated nematode management. Role of biotechnology in nematode management.
Unit 8 : Interactions of Nematodes with Soil Organisms Importance of interactions (interrelationships) of nematodes with soil organisms. Interactions of nematodes with bacteria, fungi, viruses, mycorhizae and other nematodes. Nematodes as vectors of viruses and other microorganisms.
Unit 9 : Statistics Frequency distribution. Measures of central tendency and dispersion: mean, median, mode, standard deviation etc. Population distributions : normal, binomial and poisson. Correlations: partial and multiple. Tests of significance: t, F and Chi square and randomized block, Latin square and split plot designs, their analysis and interpretation.
07. PLANT BIOCHEMISTRY
Unit 1: Basic Biochemistry and Biomolecules Scope and importance of biochemistry and molecular biology in plants. Structural and functional organization of prokaryotic and eukaryotic cells, viruses and bacteriophages, cell organelles function and their fractionation. Chemical bonding in biological systems, pH and buffers. Thermodynamics and bioenergetics- concept of entropy, and free energy changes in biological reactions, Redox reactions, Role of high energy phosphates. Biomembranes. Classification structure, chemistry, properties and function of carbohydrates, proteins, lipids and nucleic acids. Components of immune system, Prostaglandins.
Unit 2: Intermediary Metabolism Anabolism, catabolism and their regulation. Metabolism of carbohydrates – glycolitic pathway, HMP pathway, TCA cycle, glyoxylate pathway and gluconeogenesis. Biological oxidation- electron transfer and oxidative phosphorylation. Lipid metabolism, degradation and biosynthesis of fatty acids, ketogenesis and causes of ketosis. Biosynthesis of sterols and phospholipids. Amino acid metabolism – catabolism of amino acids, transamination and deamination, urea cycle, biosyntheisis of amino acids. Conversion of amino acids into bioactive compounds. Metabolism of nucleic acids-degradation and biosynthesis of purines, pyrimidines and nucleotides. Integration of carbohydrate, lipid and amino acid metabolism. Signal transduction mechanisms. Role of G-proteins, cyclic nucleotides and calcium in transduction. Disorders of lipid, carbohydrate, nucleic acid, amino acid metabolism. Inborn errors of metabolism. Secondary metabolites, biotransfermation and over expression.
Unit 3: Enzymes, Vitamins and Hormones Major classes of enzymes, general properties, kinetics, active site and its mapping, activation energy and transition state. Mechanisms of enzyme action, inhibition and activation. Coenzymes and cofactors. Isoenzymes and immobilized enzymes. Regulation of enzyme activity, allosteric regulation. Multi substrate reactions, kinetic experiments to determine the mechanism of multi substrate reactions. Isolation, purification and measurement of enzyme activity. Enzyme units. Enzyme engineering. Role of enzymes in agriculture, industry, and medicine. Structure, mode of action and metabolic functions of vitamins. Deficiency diseases associated with vitamins. General description of nature hormones and disorders associated with endocrine glands, viz. pituitary, thyroid, adrenal, pancreas and gonads. Peptide and steroid hormones. Phyto hormones – auxins, gibberellins, cytokinins, ethylene, abscisic acid and new plant bio-regulators like SA, Brassino of.
Unit 4: Molecular Biology Structure of DNA and RNA Replication, transcription and translation. Post- transcriptional and translational modifications. Transcriptional and translation control of prokaryotes and eukaryotes. Features of genetic code in prokaryotes and eukaryotes. Gene expression - operon model, induction and repression, control of gene expression in prokaryotes and eykaryotes. Chloroplant and Mitochondrial genomes. Replication of viruses. Mutagens, oncogenes and carcinogenesis. General principles of recombinant DNA technology, restriction enzymes. Methods of gene transfer-plasmid and viruses as vectors, DNA and protein sequence analysis, oligonucleotide synthesis, genomic and cDNA library construction, site-directed mutagenesis, transposon tagging, chromosome walking. Basics of genome organization and mapping, functional genomics. Gene silencing. Methods for the development of transgenic organisms. Computer application in molecular biology,
nitrogen fixation. Regulation of chloroplast gene expression. Mitochondrial control of fertility. Molecular markers in plants and their uses.
Unit 10: Plant Biotechnology / Genetic Engineering Totipotency, application of tissue culture for plant improvement, cryopreservation. Protoplasm fusion. General principles of gene cloning. Isolation and characterization of plant genes and promoters. Different methods of gene transfer –direct and vectormediated. Gene silencing. Site directed mutagenesis. Molecular analysis of transformants. Potential applications of plant genetic engineering for crop improvement – insect-pest resistance (insect, viral, fungal and bacterial diseases). Abiotic stress tolerance, herbicide resistance, storage protein quality improvement, increasing shelf- life, oil quality. Biosafety and IPR issues.
08. PLANT PATHOLOGY
Unit 1: History and Principles of Plant Pathology Milestones in phytopathology with particular reference to India. Major epidemics and their social impacts. Historical developments of chemicals, legislative, cultural and biological protection measures including classification of plant diseases. Physiologic specialization, Koch’s postulates. Growth, reproduction, survival and dispersal of plant pathogens. Factors influencing infection, colonization and development of symptoms.
Unit 2: Laboratory and Analytical Techniques Preparation and sterilization of common media. Methods of isolation of pathogens and their identification. Preservation of microorganisms in pure culture. Methods of inoculation. Measurement of plant disease. Molecular detection of pathogens in seeds and other planting materials: Nucleic acid probes, Southern, Northern and Western hybridization, ELISA, ISEM and PCR. Laboratory equipment and their use: autoclave, hot air oven, laminar flow, spectrophotometer, electrophoresis, light and electron microscopy, incubator, ultracentrifuge, ELISA Reader.
Unit 3: Physiological and Molecular Plant Pathology Altered metabolism of plants under biotic and abiotic stresses. Molecular mechanisms of pathogenesis: recognition phenomenon, penetration, invasion, primary disease determinant. Enzymes and toxins in relation to plant disease. Mechanisms of resistance. Phytoalexins. PR proteins. Antiviral proteins. SAR. HR and active oxygen radicals. Tissue culture. Somoclonal variation and somatic hybridization. Elementary genetic engineering. Management of pathogens through satellite, antisense - RNA. Ribozymes, coat protein, hypovirulence cross protection/useful genes and promoter technology biosafety and bioethics.
Unit 4: Mycology Classification of fungi. Economic mycology, edible fungi and entomogenous fungi. Mycorrhizal associations. Cell organelles, their morphology, functions and chemical composition.
Unit 5: Plant Bacteriology Identification and classification of bacteria. morphology, ultrastructure and chemical composition of prokaryotic cell in relation to function. Growth curve, nutrition and auxotrophic mutants. Resting cells in prokaryotic, elementary bacterial genetics and variability: transformation, conjugation, transduction. Biology of extra chromosomal elements: plasmid borne genes and their expression: avr, her, vie and pat genes. Bacteriophages: lytic and lysogenic cycles. Prokaryotic inhibitors and their mode of action. Economic uses of prokaryotes. Morphology, biochemical characteristics, reproduction and life cycle of phytoplasma and other fastidious prokaryotes.
Unit 6: Plant Virology Nature, composition and architecture of viruses and viroids. Properties of viruses. Variability in viruses. Satellite viruses and satellite RNA. Assay of plant viruses including biological, physical, chemical, serological and molecular methods. Conventional and biotechnological techniques used in detection and diagnosis. Behaviour of viruses in plants including infection, replication and movement. Histopathological changes induced by viruses in plants, inclusion bodies.
