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Graphical Representation - Chemical Engineering - Previous Solved Exam, Exams of Engineering Chemistry

National Center For Biological SciencesEngineering Chemistry

Main points of this exam paper are: Graphical Representation, Lineweaver Burk, Enzyme Kinetics, Rearrangement, Michaelis, Hanes-Wolf, Inhibitors

Typology: Exams

2012/2013

Uploaded on 04/01/2013

atreyi
atreyi 🇮🇳

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Name: Ke 4 Chemical Engineering 170A October 8, 2007 Midterm Exam I (100 pts) Closed Book and Closed Notes One 8.5 x 11 in. page of notes (front and back) allowed 1. Label the four phases of cell growth in the graph below (4 pts). stan oncae- Agari Ex poner thne 2. In a recent paper in Bioorg. Med. Chem. Lett. 15 (2005), Tamas Sperka and colleagues investigated beta-lactam compounds as potential inhibitors of the enzyme HIV-I-protease. One compound, 1/11a, appeared to inhibit the enzyme, giving the following Lineweaver-Burke plots for the enzymatic reaction with and without the inhibitor. Wn biter Ao ynibtor 2 4 0 4 2 3 4 41S] (rot) a) What kind of inhibition does compound I/1 1a exert on the HIV-1-protease (2 pts)? co uncompetnve Vaan & Kia ch avig€ b) Estimate Vmax for the enzymatic reaction with the inhibitor (3 pts). V4 4 Vex +4 nator. = 042 s/nM 5 Vmax = 5.33 ny 3s 1