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Here's a simple experiment to extract and visualise the DNA from kiwi fruit. Children should be supervised! 1) Prepare your equipment. You will need: - Two ...
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Extracting DNA from kiwi fruit
DNA (or D eoxyribo n ucleic A cid) is a long molecule that is found in all living things. It is the instruction manual that tells each of the cells in your body what to do. DNA determines the colour of your eyes and the colour of your hair. Here’s a simple experiment to extract and visualise the DNA from kiwi fruit.
Children should be supervised!
1) Prepare your equipment You will need:
2) Make the extraction solution DNA is tightly packaged inside the nucleus of cells. The membranes of the cell and of the nucleus are rich in fats so we can break them down using a detergent. The salt helps to get rid of the proteins that package the DNA tightly inside the nucleus.
3) Prepare your fruit mush DNA can be extracted from anything living so you could also try this experiment with strawberries or bananas. Make sure you remove the fruit skins as they are mostly dead and don’t contain DNA. The kiwi needs to be broken up so the extraction solution can get to the cells.
4) Add the extraction solution to the fruit mush In this step the detergent breaks down the cell membranes so the DNA can be released. The salt removes proteins that are bound to the DNA.
5) Filter the solution This gets rid of the fruit pulp and seeds and should leave a pure solution of DNA
6) Purifying the DNA If you want an even purer solution of DNA then we need to remove proteins that are bound to the DNA. Pineapple juice contains an enzyme