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Extracting DNA from kiwi fruit, Study notes of Genetics

Here's a simple experiment to extract and visualise the DNA from kiwi fruit. Children should be supervised! 1) Prepare your equipment. You will need: - Two ...

Typology: Study notes

2021/2022

Uploaded on 09/27/2022

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Extracting DNA from kiwi fruit
DNA (or Deoxyribonucleic Acid) is a long molecule that is found in all living things. It is the
instruction manual that tells each of the cells in your body what to do. DNA determines the
colour of your eyes and the colour of your hair. Here’s a simple experiment to extract and
visualise the DNA from kiwi fruit.
Children should be supervised!
1) Prepare your equipment
You will need:
- Two kiwis
- Pineapple juice
- Table salt
- Washing up liquid
- Cold alcohol - put in the freezer before you start
the experiment (I used surgical spirit but strong
rum also works well)
- Two small glass beakers (or plastic cups)
- Sieve
- Bowl
- Tall glass/measuring cylinder
- Kitchen Roll
- Stirring rod (or chopstick)
- Knife
- Fork
- Chopping board
2) Make the extraction solution
DNA is tightly packaged inside the nucleus of cells. The
membranes of the cell and of the nucleus are rich in fats so we
can break them down using a detergent. The salt helps to get
rid of the proteins that package the DNA tightly inside the
nucleus.
- In one of your beakers measure out about 80mls water
- Add half a teaspoon of salt and stir until dissolved
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Extracting DNA from kiwi fruit

DNA (or D eoxyribo n ucleic A cid) is a long molecule that is found in all living things. It is the instruction manual that tells each of the cells in your body what to do. DNA determines the colour of your eyes and the colour of your hair. Here’s a simple experiment to extract and visualise the DNA from kiwi fruit.

Children should be supervised!

1) Prepare your equipment You will need:

  • Two kiwis
  • Pineapple juice
  • Table salt
  • Washing up liquid
  • Cold alcohol - put in the freezer before you start the experiment (I used surgical spirit but strong rum also works well)
  • Two small glass beakers (or plastic cups)
  • Sieve
  • Bowl
  • Tall glass/measuring cylinder
  • Kitchen Roll
  • Stirring rod (or chopstick)
  • Knife
  • Fork
  • Chopping board

2) Make the extraction solution DNA is tightly packaged inside the nucleus of cells. The membranes of the cell and of the nucleus are rich in fats so we can break them down using a detergent. The salt helps to get rid of the proteins that package the DNA tightly inside the nucleus.

  • In one of your beakers measure out about 80mls water
  • Add half a teaspoon of salt and stir until dissolved
  • Add two teaspoons of washing up liquid and stir gently avoiding making too many bubbles

3) Prepare your fruit mush DNA can be extracted from anything living so you could also try this experiment with strawberries or bananas. Make sure you remove the fruit skins as they are mostly dead and don’t contain DNA. The kiwi needs to be broken up so the extraction solution can get to the cells.

  • Peel your kiwis and chop into small pieces
  • Add the chopped up kiwi to the second small beaker and use the fork to mush it up

4) Add the extraction solution to the fruit mush In this step the detergent breaks down the cell membranes so the DNA can be released. The salt removes proteins that are bound to the DNA.

  • Add your extraction solution to the kiwi mush
  • Leave at room temperature for about 20 minutes

5) Filter the solution This gets rid of the fruit pulp and seeds and should leave a pure solution of DNA

  • Put your sieve over a clean bowl and line the sieve with a few sheets of damp kitchen roll
  • Pour your green mush into the sieve carefully. Be careful not to break the kitchen roll
  • Use a fork to gently push the mixture through the sieve
  • The pulp and seeds should be left in the sieve and there should be a greenish liquid in the bowl. Transfer this to a tall glass or measuring cylinder.

6) Purifying the DNA If you want an even purer solution of DNA then we need to remove proteins that are bound to the DNA. Pineapple juice contains an enzyme