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Development of tooth, Study notes of Dental Anatomy

Stages of tooth development and root formation in detail along with pictures for explanation.

Typology: Study notes

2022/2023

Available from 08/29/2024

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groundd ๐Ÿ‡ฎ๐Ÿ‡ณ

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Introduction:
At about 6 weeks of age, the primitive oral cavity or the stomodeum develops. It is
lined by the oral ectoderm (it is a stratified squamous epithelium) with connective
tissue beneath, often referred to as ectomesenchyme. The ectomesenchyme
originates from neural crest cells which are ectodermal initially and later become
mesodermal.
โ†’ The oral ectoderm contacts the endoderm of the foregut to form the
buccopharyngeal membrane. at about 27th day of gestation this membrane
ruptures and the primitive oral cavity establishes a connection to the foregut.
At about 6th week oral ectoderm proliferate more rapidly then adjust cells.
This leads to formation of the horseshoe-shape primary epithelial band in the
location of future dental arches.
At about 7th week the primary epithelial band divides into:
1. Inner lingual process called dental lamina
2. Outer buccal process called vestibular lamina
Dental Lamina โ†’ primordial
for ectoderm portion of
deciduous teeth
Vestibular Latina โ†’ oral
vestibule between alveolar
portion of jaws and the lips I
cheeks
Future
Dental lamina is towards the inner side and the
one on the outer side is the vestibular lamina.
Vestibular lamina is also called the lip furrow band
as it separates the lips and the teeth.
As the lamina continues to grow deep in the
connective tissue, the cells also undergo
degeneration to form a furrow.
The dental lamina shows increased cell growth in
certain positions forming a localised thickening called
enamel organ.
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Introduction:

At about 6 weeks of age, the primitive oral cavity or the stomodeum develops. It is lined by the oral ectoderm (it is a stratified squamous epithelium) with connective tissue beneath, often referred to as ectomesenchyme. The ectomesenchyme originates from neural crest cells which are ectodermal initially and later become mesodermal. โ†’ The oral ectoderm contacts the endoderm of the foregut to form the buccopharyngeal membrane. at about 27th day of gestation this membrane ruptures and the primitive oral cavity establishes a connection to the foregut. At about 6th week oral ectoderm proliferate more rapidly then adjust cells. This leads to formation of the horseshoe-shape primary epithelial band in the location of future dental arches. Dental Lamina โ†’ primordial for ectoderm portion of deciduous teeth Vestibular Latina โ†’ oral vestibule between alveolar portion of jaws and the lips I cheeks Future Dental lamina is towards the inner side and the one on the outer side is the vestibular lamina. Vestibular lamina is also called the lip furrow band as it separates the lips and the teeth. As the lamina continues to grow deep in the connective tissue, the cells also undergo degeneration to form a furrow. The dental lamina shows increased cell growth in certain positions forming a localised thickening called enamel organ.

โ†’ Dental lamina is the structure that gives rise to all the deciduous teeth, which later extends to form distal extension which gives rise to 3 molars. Successional lamina gives rise to permanent teeth โ†’ oral ectoderm shows proliferation of cells into the connective tissue to form the dental lamina, proceeded by proliferation of dental lamina cells to form the enamel organ. โ†’ Enamel organ is round and oval in shape. โ†’ A basement membrane separates the enamel organ from the surrounding connective tissue. โ†’ types of cells that are seen in the enamel organ are: a. The central cells that are polygonal b. The peripheral cells that are cuboidal or low columnar โ†’ Morphodifferentiation of enamel organ is seen but NO HISTODIFFERENTIATION. ie. change in shape - yes Specialisation of cells - no โ†’ As the enamel organ forms, the ectomesenchymal cells start surrounding it, which is called ectomesenchymal condensation. This happens due to a reasons: a. Increased mitosis of cells near the enamel organ. b. migration of cells adjacent to the enamel organ. morphological stages

  1. levd^ stage

โ†’ As the enamel organ grows in size, it attains a concavity which gives it an appearance similar to a cap. This happens because of unequal grow in different parts of the enamel organ. โ†’ Morphodifferentiation- yes Histodifferentiation - yes โ†’ 3 types of cells are seen in the cap stage: a. single layer of cells outlining the convex surface โ†’ OUTER ENAMEL EPITHELIUM (cuboidal in shape) b. single layer of cells outlining the concave surface โ†’ INNER ENAMEL EPITHELIUM (later differentiate into tall columnar in shapes ameloblast and form enamel. ) c. cells present in between are called โ†’ STELLATE RETICULUM as they are star shaped. โ†’ why the formation of STAR SHAPED CELLS?? This is because the central cells start releasing glycosaminoglycans (GAG) into the intercellular area. โ†“ GAG is hydrophilic, hence attract water from the surrounding tissue โ†“ This water pushes the central cells apart but they are still connected by intercellular bridges. โ†“ gives an appearance of star shaped cells. โ†’ stellate reticulum due to presence of water has a cushion-like consistency. The function of stellate reticulum is to protect inner enamel epithelium (shock absorber)

  1. (^) Cafe stage -^ >

โ†’ The junction of inner and outer enamel epithelium is called the cervical loop which is an area of intense mitotic activity. (size increase in this direction) โ†’ The dental lamina still attaches the enamel organ with oral ectoderm. sometimes, two or more dental lamina help attach enamel organ which has entrapped ectomesenchyme called ENAMEL NICHE. โ†’ TRANSIENT/temporary structures of enamel organ: disappear before enamel formation.

  1. Enamel KNOT (dense collection of cells in stellate reticulum just dividing cells above IEE)
  2. Enamel CORD (extension of cells towards OEE)
  3. Enamel SEPTUM (extension of cells reaches OEE)
  4. Enamel NAVEL (concavity on OEE where enamel septum makes contact. ) Enamel knot behaves as signalling centre and decides shape of tooth to be formed. โ†’ condensed ectomesenchyme can now be differentiated: a. within the concavity and below it โ†’ DENTAL PAPILLA b. surrounding enamel organ and papilla โ†’ DENTAL SAC IEE - inner enamel epithelium OEE- outer enamel epithelium
  5. Outer enamel epithelium
  6. Stellate reticulum Dental papilla Dental sac Enamel septum, cord and knot are reservoirs of dividing cells note

Stratum intermedium โ†‘

  • new (^) layer

โ†’ By the end of early bell stage, the IEE cells differentiate into ameloblasts, which are tall columnar 210M high, 1 M width โ†’ Nucleus of ameloblast shifts away from papilla and towards stellate reticulum, which is called reversal of polarity. seen in odontoblast as well. โ†’ differentiation of IEE into ameloblast starts at cuspal/incisal tips and then proceeds cervically โ†’ formation of ameloblast sends signal to DENTAL PAPILLA โ†“ which causes cells present adjacent to IEE in ectomesenchyme to differentiate into odontoblast which eliminates acellular zone odontoblast starts depositing dentin first. โ†“ After formation of one layer of dentin, enamel formation is initiated by ameloblast โ†’ In advanced bell stage, the nutrients are not able to cross the mineralised wall near dental papilla to IEE. Hence, ameloblast derive nutrition from dental sac. As the distance blw them is large so stellate reticulum goes under gradual degeneration to reduce distance. OEE cells become low cuboidal in shape. โ†’ is proximation of blood vessels near the outer enamel epithelium folds so that the ameloblast can derive nutrition.

4. advance hell^ stage -

  1. initiation
  2. Proliferation
  3. Histo differentiation
  4. Morphodifferentiation
  5. Apposition โ†’ Initiation is the shortest phase. It begins with the proliferation of the dental lamina leading to the formation of dental placodes. This process requires epithelial -mesenchymal interaction. โ†’ proliferation is the longest period. Enamel organ only stops growing after the formation of ameloblast cells. โ†’ Histo-differentiation begins in the cap stage when the inner enamel epithelial start undergoing changes to form ameloblast. in cap stage -formation of stellate reticulum, in bell stage - formation of stratum intermedium in advanced bell stage forma -tion of amebblast and odontoblast. โ†’ Apposition: deposition of minerals. โ†’ morphodifferentiation occurs in all the stages. physiological stages - /

โ†’ once mineralisation reaches the cervical loop then the root formation begins by formation of Hertwigs Epithelial Rooth sheath which moves aplically. Root formation begins at advanced bell stage. โ†’ The Hertwigs Epithelial Rooth sheath is bilayer in nature. It grows from the cervical loop into the underlaying ectomesenchyme, separating the dental papilla from the dental sac. HERS is formed by extension of cells from cervical loop, it has inner enamel epithelium and the outer enamel epithelium cells but there no stellate reticulum or stratum intermedium. Both the inner and outer layer are cuboidal in nature. โ†’ HERs sends a signal to the dental papilla, which differentiates the cells into odontoblasts, which start depositing dentin. once deposition of dentin starts the work of HERS is done hence it undergoes degeneration. A few remanants might be there which is called cell rests of malasses. (which are dormant in nature with no function but may give rise to pathologies like tumour or cysts.) They are found in peridontal ligaments, towards cementum. Due to degeneration of HERS the cells of dental sac come in contact with dentin which causes them to differentiate into cementoblasts for cementum deposition. โ†’ HERs bends and grows horizontally to form an epithelial diaphragm, this diaphgram does not meet in the middle and so reduces the size of the cervical opening to form the apical foramen. In multi-rooted tooth the Epithelial diaphragm grows in extensions to form 2/ 3 cavical openings which gives rise to multiple roots. root (^) formation