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BG416 Biotechnologies Time allowed: Three Hours Semester 1 Examinations 2010/ Exam Code(s) 4BY Exam(s) Module Code(s) BG Module(s) Biotechnologies Paper No. 1 Repeat Paper External Examiner(s) Prof. M. J. R. Stark Internal Examiner(s) Dr A. Flaus, Dr P. Creighton, Dr M. Tuohy, Dr L. Howard, Prof R. Ceredig, Dr U. Greiser, Prof K. Sullivan Instructions: Answer three questions. Please start each answer with a new answer book and clearly indicate the number on the front. All questions carry equal marks. Duration 3 hours No. of Pages 3 Discipline(s) Biochemistry Course Co-ordinator(s) Dr. Andrew Flaus Requirements : MCQ Release to Library: Yes x No Handout Statistical/ Log Tables Cambridge Tables Graph Paper Log Graph Paper Other Materials
Write on three of the following topics, choosing at least one from each section. Please start each answer with a new answer book and clearly indicate the number on the front. Section A
1. List the components for a standard PCR reaction, and describe the standard PCR method. Explain how the components and method is extended in both the RT-PCR and qPCR. Show diagrams of example results expected for all three methods. 2. Describe the antibody phage display technology, it's principles, advantages and disadvantages compared to other antibody technologies. Explain its application in academia and industry. 3. ‘Recent developments in DNA sequencing technologies will facilitate a new era of personal genomics’. Explain this statement, by describing some of the new sequencing strategies which have been developed. Section B 4. Describe a typical proteomics strategy based on the combination of multi- dimensional separation techniques with mass spectrometry. How would you obtain ‘de novo’ sequence and/or peptide mass fingerprint data for a protein? 5. You are examining a protein, Ace, that translocates from the cytoplasm to a discrete compartment in the nucleus where you suspect it binds to a kinase named Xam-1. Discuss how you would use GFP fusion proteins and live cell microscopy to measure two specific aspects of the pathway involving Ace and Xam- 1. 6. High performance anion exchange chromatography (HPAEC) is a specialized type of high performance liquid chromatography (HPLC). Describe its use in the separation and analysis of carbohydrates.
