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SPRING Examinations 2009 / 2010
Exam Code(s) 1MT Exam(s) M.Sc. Degree (Biotechnology) Module Code(s) BG Module(s) Biotechnology II Paper No. 2 External Examiner(s) Dr. John P. Quinn Internal Examiner(s) Professor Vincent O’Flaherty *Dr. Aoife Boyd Instructions Answer 4 Questions in total. TWO questions from Section A & TWO questions from Section B Use a separate answer book for each Section Please indicate clearly the numbers of the questions answered on the first page of your Answer Book Duration (^) 3 Hours No. of Pages 1 Department(s) Microbiology Co-ordinator Requirements MCQ Release to Library: Yes √ No Handout Statistical/Log Tables Cambridge Tables Graph Paper Log Graph Paper Other Materials
SECTION A
1. Answer EITHER (i) Discuss the advantages and disadvantages of continuous culture systems for industrial bioprocesses. OR (ii) Write an essay on fed-batch cultures. What advantages do they have for the industrial microbiologist? 2. Answer EITHER (i)Write an essay on the importance of aeration and agitation in the operation of fermenters. OR (ii) Discuss the problems encountered in sterilising media in large-scale fermenters.
SECTION B
3. Describe DNA probe based and molecular genotyping methods and outline some applications of these methods.
4. Describe how you would design a PCR test and outline some applications of PCR- based tests in either the food or healthcare sectors. 5. Write short notes on four of the following: (i) Nucleic acid extraction methods (ii) Microarray technology (iii) Molecular methods in the BioIndustry sector (iv) Real-time PCR technology (v) In-vitro amplification methods (vi) Advantages of molecular methods compared to microbiological methods 6. "The structure of DNA provides the basis for recombinant technologies". Discuss.
